Single Cell Isolation Microfluidics

Many single cell analysis techniques require the isolation of individual cells.

Single cell isolation microfluidics.

Methods currently used for single cell isolation include. The throughput of this method was later increased to 10 6 cells by single cell isolation using droplet microfluidics instead of compartmentalization in microwells. Single cell analysis has become one of the most important applications of microfluidic technology in recent years. Using a hybrid microfluidics method droplets in channels can be individually addressed using a co planar electrode system.

We demonstrate the benefit of this approach to screen antibodies for the specific binding of cancer target cells in a high throughput single cell format which should have immense clinical potential. The unique droplet microfluidic technology speeds up the process at the step of isolating activated single antibody secreting cells in picoliter volume chambers from various tissues including blood bone marrow and b cell plasma which allows secreted antibodies to be detected within hours with a variety of bead based and cell based assay formats. 133 134 abvitro later acquired by juno therapeutics developed a similar approach for sequencing. Depending on the application single cell analysis chips can include any or all the above mentioned steps.

In this work a microfluidic method that is capable of isolating single cells in droplets selecting successful edited clones and expansion of these isoclones is introduced. 131 132 in parallel this technology has been developed commercially by gigagen.